By Jack G. Chirikjian
Vol. I: The paintings provided in those volumes is the collaborative attempt of over twenty undergraduate technological know-how college, whose universal aim used to be to boost a textual content of exact and versatile laboratory actions focusing at the concept and perform of biotechnology for undergraduate scholars. The books are designed to supply flexibility for simple integration into any direction within the existence sciences with an experimental emphasis.
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Extra resources for Biotechnology: Plant biotechnology, animal cell culture, immunobiotechnology
Precautions should be taken to avoid igniting hair or clothing. Experimental Outline Disinfect plant tissue and prepare explants. Inoculate medium with plant tissue. Observe cultures over a period of several weeks. Materials Sterile media (this has been prepared prior to class) Plant material 10% Household bleach solution (prepare fresh) Sterile Water 70% Ethanol 95% Ethanol Forceps Scalpel Page 8 Pre-lab Preparation Tissue culture medium should be prepared in advance of the laboratory period. This can be done several days to a week ahead of time.
3. Wash the explants in a beaker of distilled water to which you have added a few drops of detergent. 4. Using forceps, transfer the explants to a 70% solution of ethanol for 2 minutes. 5. Again using forceps, transfer the explants to a 10% solution of household bleach for 510 minutes. Tender leaf tissue should not be left in the bleach for more than about 5 minutes. Root or stem pieces can be left for longer times. 6. After treatment with bleach, the tissue is considered to be sterile. Care should be taken to avoid re-contaminating it.
Holland Salisbury State University Salisbury, Maryland Barbara Jones Southwestern Adventist College Keene, Texas Baldwin King Drew University Madison, New Jersey Edward Kisailus Canisius College Buffalo, New York Karen K. Klyczek University of Wisconsin-River Falls River Falls, Wisconsin Robert Krasner Providence College Providence, Rhode Island Malethu T. Mathew Virginia Union University Richmond, Virginia Harley Mortense SW Missouri State University Springfield, Missouri Ah-Kau Ng University of Southern Maine Portland, Maine Mark Petersen Blue Mountain Community College Pendleton, Oregon E.