Download Biotechnology: Plant biotechnology, animal cell culture, by Jack G. Chirikjian PDF

By Jack G. Chirikjian

Vol. I: The paintings provided in those volumes is the collaborative attempt of over twenty undergraduate technological know-how college, whose universal aim used to be to boost a textual content of exact and versatile laboratory actions focusing at the concept and perform of biotechnology for undergraduate scholars. The books are designed to supply flexibility for simple integration into any direction within the existence sciences with an experimental emphasis.

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Extra resources for Biotechnology: Plant biotechnology, animal cell culture, immunobiotechnology

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Precautions should be taken to avoid igniting hair or clothing. Experimental Outline Disinfect plant tissue and prepare explants. Inoculate medium with plant tissue. Observe cultures over a period of several weeks. Materials Sterile media (this has been prepared prior to class) Plant material 10% Household bleach solution (prepare fresh) Sterile Water 70% Ethanol 95% Ethanol Forceps Scalpel Page 8 Pre-lab Preparation Tissue culture medium should be prepared in advance of the laboratory period. This can be done several days to a week ahead of time.

3. Wash the explants in a beaker of distilled water to which you have added a few drops of detergent. 4. Using forceps, transfer the explants to a 70% solution of ethanol for 2 minutes. 5. Again using forceps, transfer the explants to a 10% solution of household bleach for 510 minutes. Tender leaf tissue should not be left in the bleach for more than about 5 minutes. Root or stem pieces can be left for longer times. 6. After treatment with bleach, the tissue is considered to be sterile. Care should be taken to avoid re-contaminating it.

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